We developed a biosensor using Pyrococcus furiosus Argonaute (PfAgo) for the highly sensitive and specific detection of single-nucleotide mutations causing fungicide resistance. This PfAgo-based dual-recognition method achieved a detection limit of 0.1 fM and was successfully validated in real samples of fungal pathogens.

Using CUT&Tag sequencing, we mapped the genome-wide binding sites of the transcription factor MEF2A in chicken primary myoblasts, identifying 1,768 target genes involved in myogenesis. We further demonstrated that MEF2A binds to the MYL2 promoter to activate its transcription, providing new insights into the molecular mechanisms of chicken muscle development.

We investigated the tissue expression and functional characteristics of the calcitonin (CT) family peptides and their receptors in chickens. Our findings reveal distinct physiological roles for the CT/CTR and CGRP/CLR/RAMP1 signaling pathways, with CGRP showing a potential role in regulating glucose metabolism in the chicken liver.